NIR instruments working in
reflectance analyze first a reference (you can consider it as a blank) and
after, the reference move from the path and the light goes to the sample to get
an spectrum corrected versus the reference. So the reference is an important
part of the instrument, so we must check their performance in the future
respect to the present or the past.
In a spectrum of the reference
we have included many things, like the state of the optics, the detector´s
response, state of the lamp,.....
In some instruments the
reference is exposed to external conditions (humidity, dust,...) more than
others. NIR Companies are conscious that the reference spectra must be as much
stable as possible during the NIR instrument life, so now the reference
ceramics are more protected and they are more stable all along the instrument
life.
An external
reference will help us to check the spectrum of the internal reference, and correct, when necessary, the differences. This external reference has a
file with the spectra of the difference between the external reference
itself and a master standard. So all the instruments can be reference corrected
to have the same reference spectrum. This is known also as reference
standardization or External Reference Correction. This technique will help to transfer in a better way the calibrations from an instrument to other.
An example is after an
instrument repair (changing for example the monochromator which include the
optics and detectors), if we dont run the ERC the spectrum of a sample, like the
Check Cell (which is quite stable over time), will look different before and
after the repair, and the predictions are quite different for some of the
parameters. If we scan the external reference and correct the spectrum using
the file, the spectrum of the check cell will change to overlap their shape
previously to the repair (same for the predictions).
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